DOI: https://dx.doi.org/10.18565/pharmateca.2018.6.60-65

V.V. Ostromensky (1), M.K. Astapova (2), G.B. Dikke (3), Yu.G. Kucheryavaya (1), D.N. Perunova (4), P.V. Antonov (5)

1 Maternity Hospital № 2, St. Petersburg, Russia; 2 North-Western State Medical University n.a. I.I. Mechnikov, Saint-Petersburg, Russia; 3 RUDN University, Moscow, Russia; 4 St. Petersburg State Pediatric Medical University, St. Petersburg, Russia; 5 St. Petersburg State University, St. Petersburg, Russia
Objective: to compare tissue effects in the wound area on the uterus after applying argon-plasma coagulation (APC) using Fotek ЕА141 and the injection of hyaluronic acid. Methods. An experimental study on animals was carried out. The study involved 30 female rats. On the 14th day after the surgery, scars of the left uterine horn treated with APC (n=15 – group 1) or injection of 1.0 ml hyaluronic acid (n=15 – group 2) were examined. To evaluate the structure of the scar, a histological examination of sections with Van Gieson staining was performed. Control – scars of right uterine horn (n=30). Evaluation of the results of the study was carried out using the developed grading scale of histological changes. Results. Comparison of the methods studied has revealed a more pronounced effect of APC on angiogenesis in the wound area. At the same time, the injection of hyaluronic acid has increased the number of myocytes in the scar. Evaluation of the histological structure of the sections showed that the mean scar score in the group 1 was 10.8±1.28 points, in group 2 – 11.2±1.32, and was better than in the control (8.0±1.16) as expected. Moreover, this pattern was noted both in the whole group and in comparison of the scar on the right and left horns of the uterus in the same individual. Scars in the studied groups were histologically regarded as «small, with a noticeable muscle ingrowth» or as a «scar with a large amount of muscle tissue», whereas in the control group «dense, rough scars» or «scars with a predominant connective tissue» predominated. Conclusion. The positive effects of both methods on myometrium can be used to form a regular scar after surgery on the uterus.

About the Autors

Corresponding author: V.V. Ostromensky – PhD, Deputy Chief Physician for Medical Work, Medical and Prophylactic Institution «Maternity Hospital
№ 2», St. Petersburg, Russia; e-mail: ostromenskyvv@gmail.com

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