Evaluation of the ability of benzydamine hydrochloride to inhibit planktonic cells, as well as growing and mature biofilms of clinically important microorganisms

DOI: https://dx.doi.org/10.18565/pharmateca.2021.1.102-107

P.V. Slukin (1), N.K. Fursova (1), I.V. Kukes (2, 3), N.I. Briko (4)

1) State Research Center for Applied Microbiology and Biotechnology, Obolensk, Serpukhov, Moscow Region, Russia; 2) Angelini Pharma Rus LLC, Moscow, Russia; 3) International Association of Clinical Pharmacologists and Pharmacists, Moscow, Russia; 4) I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia
Background. Biofilms are a huge healthcare problem around the world because these formations provide additional opportunities for bacteria and fungi to resist antimicrobial drugs. Nowadays, a large range of antimicrobial drugs have been registered in Russia, however, evidence of their antagonistic activity against biofilms is limited. Benzydamine is a popular and actual local anti-inflammatory drug with antimicrobial properties that is why this is a high interest to test it against biofilms.
Objective. To form a collection of clinical isolates (gram-positive bacteria, gram-negative bacteria and yeast-like fungi), to study the sensitivity of their planktonic and biofilm cultures to the antimicrobial drug benzydamine hydrochloride.
Methods. Determination of the minimum inhibitory concentrations of benzydamine hydrochloride against test-cultures of microorganisms by the method of serial dilutions in liquid and solid nutrient media.
Results. A collection of strains has been created. New data have been obtained on the sensitivity of clinically significant microorganisms to benzydamine hydrochloride.
Conclusions. It has been shown that planktonic cultures and biofilm-forming test-strains of Pseudomonas aeruginosa, Moraxella catarrhalis, Haemophilus influenzae, Gardnerella vaginalis, Staphylococcus spp., Streptococcus pneumoniae, Candida spp. are sensitive to benzydamine hydrochloride at a concentration corresponding to that in Tantum® Verde 0,15 and 0,3%. The inhibitory activity of benzydamine hydrochloride against mature biofilms of clinically significant microorganisms was also noted in the concentration range 160–1280 mg/l.
Keywords: benzydamine hydrochloride, antibacterial activity, minimum inhibitory concentration, clinical strains, biofilms

About the Autors

Corresponding author: Nadezhda K. Fursova, Cand. Sci. (Biol.), Leading Researcher, Antimicrobial Agents Laboratory, Department of Molecular Microbiology, State Research Center for Applied Microbiology and Biotechnology, Moscow Region, Russia; n-fursova@yandex.ru
Address: 24 “Quarter A” Territory, 142279 Obolensk, City District Serpukhov, Moscow Region, Russian Federation 

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